Coding
Part:BBa_K242101:Design
Designed by: Laura Gomez, Uriel Urquiza Group: iGEM09_LCG-UNAM-Mexico (2009-10-20)
GFP induced by T7/T3 RNA polymerases
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 726
Design Notes
We want to have a reporter when the phage infects our cells.
Source
Other biobricks
References
Nucleic Acids Res. 1992 May 25; 20(10): 2517–2524.
PMCID: PMC312387 Copyright notice T7 promoter contacts essential for promoter activity in vivo. R A Ikeda, C M Ligman, and S Warshamana